ABSTRACT
Objective: to identify the frequency of the rs143383 SNP in the GDF5 gene, which is located in the 5'-untranslated region of Turkish population with knee osteoarthritis [OA]
Study Design: a case-control study
Place and Duration of Study: orthopedics and Traumatology Department, Bozok University Medical Faculty, Yozgat, Turkey, from 2012 to 2014
Methodology: patients diagnosed with OA [n=94] and patients who did not have joint complaints [n=279] were enrolled in this study. Patients diagnosed with osteoarthritis according to the 1986 American College of Rheumatology osteoarthritis criteria and Kellgren and Lawrence scores were investigated, based on age, gender, and X-ray findings. Blood samples were taken for the identification of GDF5 [rs143383] SNPs by PCR/RFLP, according to a standard protocol
Results: this study included 373 patients. The OA group [25.2%; n=94] was characterized by specific genotypes: TT [39.4%; n=37]; heterozygotes [TC; 45.7%; n=43]; and homozygotes [CC; 14.9%; n=14]. The control group [74.8%; n=279] was comprised of TT [26.5%; n=74], TC [54.8%; n=153], and CC [18.6%; n=52] genotypes. An analysis of rs143383 SNP of the GDF5 gene polymorphism revealed that the rs143383 TT genotype had a higher risk for OA [crude OR=1.798, 95% CI=1.010-2.941, p=0.021]
Conclusion: this study demonstrated that there is a correlation of +104T/C polymorphism in the 5'-UTR of GDF5 with knee OA in a Turkish population
ABSTRACT
There are several types of treatment modalities for wrist ganglions. The aim of the study was to assess the effectiveness of cyst aspiration and methyl prednisolone acetate injection with double IV cannula rather than sharp pointed needle, as a new technique in the treatment of volar ganglia. The study involves total of 19 patients who received treatment by aspiration and methyl prednisolone injection into the cavity. Two IV cannulas are pricked to the cystic cavity. Cyst fluid is drained by the distally placed IV cannula meanwhile injecting methyl prednisolone by proximally placed IV cannula. The patient records and follow-up reports are retrospectively investigated. The patient age, sex, site of the cyst, the treatment that was applied, adjacency to the artery and the nerves and recurrence are recorded. Mean follow up time was 2.1 +/- 0.5 years. The study involved 19 patients that received aspiration treatment for volar ganglion cysts between January 2004 and December 2009. There were 12 [63.2%] female and 7 [36.8%] male subject with volar wrist ganglion cyst. The mean age of patients was 27.63 +/- 6.6 years. Fourteen [73.7%] patients of total had cysts close to the artery. We didn't observe any complication related to methyl prednisolone injection and arterial ischemia. Recurrence was observed in three [15.8%] patients. This method has lower recurrence rate than other aspiration therapy with sharp pointed needle. We prefer to use IV cannula needle for cyst aspiration and steroid injection in treatment of volar ganglia before any surgical intervention
Subject(s)
Humans , Male , Female , Wrist , Palmar Plate , Suction , Methylprednisolone , CathetersABSTRACT
Evaluation of anti-inflammatory effect of Glucosamine sulfate [GS] versus diclofenac sodium [DS] in effusion of osteoarthritic knees. In this study, patients were included in this study from 2007-2010 based on American College of Rheumatology criteria with OA and physical examination in effusion of osteoarthritic knees. The patients were divided into two groups. First group [27 patients] DS was given in doses 75 mg twice daily for ten day. In the group II [25 patients] GS was used in doses of 1500 mg two times daily over the first 12 weeks of the study. A closed aspiration was performed. The knee circumference was measured in patients before and 12 week after treatment. Before and after 12 weeks of treatments, both groups of patients were assessed according to the WOMAC questionnaire of knee pain and function scores. Comparison of knee mean circumference between the two groups was not statistically significant before treatment [p=0.938], but significant after treatment [p < 0.001]. At the end of the 12 week, there was 66.6% complete resolution of effusion in the DS group [18 patients] and 24.0% [6 patients] in the GS group, this was statistically significant [P < 0.001]. DS groups, results of the beginning and at the end of 12 week measurement showed significant differences in WOMAC pain mean score [P < 0.001] but GS groups not statistically significant [P=0.160]. The WOMAC function mean scores in pre and post-treatment periods of follow-up showed significant variation between the two groups [P < 0.001, P < 0.001]. Our observations suggest that GS is not able to suppress the progression of adjuvant arthritis in OA with effusion of knee osteoarthritis. GS should not be expected as anti-inflammatory influence as DF in the treatment of OA-related effusion